Lego Batman 2: DC Super Heroes features an open world setting, in a similar gameplay style of the Batman: Arkham series, where full exploration of Gotham City is granted.

The gameplay is similar to its predecessor, Lego Batman: The Videogame, but allows players to play as classic Batman characters and other various DC Comics superheroes, such as Robin, Batgirl, Green Lantern, Superman, The Flash, and Wonder Woman.

There are 75 playable characters.

New gadgets such as Robin's Hazard Cannon, Green Lantern's Power Ring, Superman's heat vision, and a Power Suit for Batman were introduced.

An optional 2-player cooperative play mode is also present.