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Retrograde Cellular Transport ofHerpes Simplex Virus: Interactions between Viral and Motor Proteins - a Gene Therapy Vector for Spinal Cord Disease?

Retrograde Cellular Transport ofHerpes Simplex Virus: Interactions between Viral and Motor Proteins - a Gene Therapy Vector for Spinal Cord Disease?

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    Retrograde Cellular Transport ofHerpes Simplex Virus: Interactions between Viral and Motor Proteins - a Gene Therapy Vector for Spinal Cord Disease?

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    Herpes simplex virus type 1 (HSV-1) establishes life-long latent infection in sensory neurones, making it an ideal gene therapy vector to target neuronal cells.To study retrograde transport of HSV-1, capsid and tegument proteins were tested for interactions with the motor cytoplasmic dynein, using a yeast two-hybrid system and in vitro pull down assays.

    A strong interaction was demonstrated between the HSV-1 outer capsid protein VP26 (UL35) and the homologous 14 kDa dynein light chains RP3 and Tctex1.

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